Hardware
InSight allows realtime 3D acquisition using a scanless method based on a proprietary light field technique. InSight is a flexible imaging device adding light field imaging capabilities including one shot 3D acquisition, parallax view and digital refocusing as well as fast Z-scanning capabilities

Software
InSight is supplied with GUI software QTImage. An optional API/ SDK is provided for microscopy automated systems with comprehensive 3D acquisition tools.

Applications include

• High-speed volumetric imaging of weak fluorescent specimens
• In-vivo imaging of C. elegans, Drosophila and zebrafish
• 3D functional imaging of neuronal activity
• High-speed Ca2+ imaging
• Fluorescence and widefield microscopy
• Microfluidics
• live Imaging of cellular dynamics in three dimensions
• Trajectory monitoring of microscopic objects
• Instant 3D recording of dynamic events or moving specimens

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InSight LFM (add-on for any microscope)

Camera compatibility (camera not included)	Format >=1” (see compatibility list)
Microscope Interface	Video Port 1X C-mount adapter
Physical Dimensions (mm), Weight (g)	InSight Head: 245(H) 56(W) 80(D), 470 g Control Unit: 40(H) 160(W) 150(D), 150 g

InSight LFC (with embedded camera)

Camera	Scientific CMOS 13.31 mm x 13.31 mm
Sensor specs	2048 x 2048 6.5 µm x 6.5 µm
Full well capacity	30 000 electrons
Dynamic range	33000:1
Frame rate (full resolution)	Frame rate (full resolution)

Scanless Mode (Light Field)

Z range and Z resolution are objective dependant
Z Range = 100 x Objective Depth of Field
Z Resolution = Z Range / 10

Scanning Mode

Z range and Z step are objective dependant
Z Range = 16mm / (G_Obj)²
Z Step = Z Range / 2000

InSight LFM is supplied with the Graphics User Interface “Qtimage”, touch screen compatible, Windows 8, 7, Vista , XP  (32/64 bits)

QTIimage  GUI (included)

Main Features:
Deconvolution
Time Lapse
Multi Focus Image
Export to 3rd party software

LightField mode:
Onde shot / real tme 3D acquisition
Parallax mode
Digital refocusing,
Z-stack

Z-Scanning mode:
Z-stacking
DIC, Phase, using brightfield objectives
Digital Refocusing

System_Calibration software (Included)

Main features:
XY calibration for each objective magnification
Z-stack alignment parameters calculation
Calibration data are stored in acquisition system memory

API / SDK (Optional)

An optional API/ SDK is provided for microscopy automated systems with comprehensive 3D acquisition tools.