
| ZeeScope 100 | ZeeScope 150 | ZeeScope 200 | |
| Camera |
CMOS 1280 x 1024 5.2?m square pixels 30fps@full resolution |
CCD 1616 x 1216 4.40 square pixels 12fps@full resolution |
CCD 2560 x 1920 2.20 square pixels 6fps@full resolution |
| Light source | Built-in LED light source | ||
| Objectives |
Interchangeable objectives (Finite – Infinite type) adapter provided for threads RMS and M25/0.75 |
||
|
Dimensions & weight ZeeScope Head Control unit |
225(H) x 40 (W) x 55(D) mm, 425g | ||
| Power supply | 110/220V AC | ||
| PC interface | USB 2.0 | ||
Z range and resolution are objective and c-mount coupler magnification dependant. The table here under gives typical performance for standard objective magnification. For any other magnification, the following formulas can be applied:
Z Range = 60mm / (G_Obj )2
Z Resolution = Objective Depth Of Field /4
G_Obg = Objective magnification
|
Objective Mag : NA |
Z Range (?m) | Z Resolution (?m) |
| 5X / 0.10 | 2400 | 18.5 |
| 10X / 0.25 | 600 | 3 |
| 20X / 0.45 | 150 | 1 |
| 50X / 0.8 | 24 | 0.25 |
Z accuracy: 1%
Z Repeatability: 0.35%
Max slope: 90°
XY Spatial resolution determined by camera resolution and objective magnification
12 analysis parameters are provided in total, including the frequently-used Ra (Sa), Rq (Sq), Rz (Sz), parameters. Parameters conform to ISO 4287, 25178 DIN 4768
Measuring range: Ra, Rq: 0.01-500µm Measuring accuracy: ?±10% Repeatability: Less than or equal to 6%
Field of View (FOV) = (Camera (H/V) resolution * camera pixel (H/V) / (G_Obj * 0.537)
Depth of Field (DOF) = lambda / NA + pixelsize / (NA? * G_Obj * 0.537)
G_Obj = objective magnification
G_adapt = c-mount coupler magnification
Lambda = typically 0.633µm
NA = objective numerical aperture
Pixelsize = camera pixel size
Calibration for the Z axis is performed in Phaseview factory before shipment, using step height certified calibration standard. Checking Z calibration can be easily achieved using step height calibration standard such as Rubert satndard item number 513C (optional)
A system calibration software (see software specifications) is provided for XY calibration according to objective magnification and calculation of corrected XY shift parameters to be applied along the Z axis (Z-stack). The calibration data are then stored in an internal memory of the acquisition system. Typical accessories required for the calibration (optional).
ZeeScope is supplied with the Graphics User Interface « GetPhase « and a System Calibration software. An optional API / SDK is provided for integration purpose. All software are XP, Vista, 7, 8 (32 / 64 bit) compatible, and Graphics Processor Units (GPU) compatible.
Main features:
2D / 3D acquisition modes: 2D, Z-stack, 3D roughness, 3D Shape
Multiple display modes : 3D, Phase, DIC, Darkfield, Image fusion (Extended Depth of Field)
2D / 3D analysis tools : profiles, step height, roughness, etc.
Report & Export Data
GetPhase includes 3 different methods for Z axis and 3D acquisition:
Z Height measurement / Z-Stacking: relies on the unique Z-scanning capability of the PhaseView optical assembly providing accurate and repeatable Z steps.
3D Roughness Measurement: relies on proprietary wavefront technique, for measuring surface topography in reflection. The algorithm processes a set of 2 or more images acquired within objective depth of field. This method is particularly useful for measuring small surface variations, when sample features are all in-focus, within depth of fiel of the selected objective. The 3D reconstruction is determined by the maximum slope constraint; samples with steep slopes require high objective magnification as related to objective numerical aperture (NA).
3D Shape Measurement: relies on detecting local contrast on Z image series for measuring depth map of an object. The algorithm processes Z-stack images acquired beyond the objective depth of field. This method is well adapted to samples having surface variations beyond objective depth of field. 3D reconstruction is performed when samples exhibit some texture along the Z image planes.
Main features:
XY calibration for each objective magnification
Z-stack alignment parameters calculation
Calibration data are stored in acquisition system memory
An optional API/ SDK is provided for microscopy automated systems with comprehensive 3D acquisition tools.